|
| Status |
Public on Mar 28, 2013 |
| Title |
WT vs. MO-grnA injected, replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
MO-grnA morphant
|
| Organism |
Danio rerio |
| Characteristics |
age: 22 hpf
treatment: MO-grnA
|
| Treatment protocol |
An injection volume from 2-3 nl of MOs was injected into 1-cell-stage embryos.
|
| Growth protocol |
The zebrafish were maintained at our own facility in a controlled environment with a 14/10-h light-dark cycle at 28 °C. The fertilized eggs were collected at the 1-cell stage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following the manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Agilent's Quick Amp Labeling Kit generates fluorescent cRNA with a sample input RNA 1 μg of total RNA for two-color processing.The method uses T7 RNA polymerase,which simultaneously amplifies target material and incorporates cyanine 3- or cyanine 5-labeled CTP.
|
| |
|
| Channel 2 |
| Source name |
WT embryo
|
| Organism |
Danio rerio |
| Characteristics |
age: 22 hpf
treatment: none
|
| Treatment protocol |
An injection volume from 2-3 nl of MOs was injected into 1-cell-stage embryos.
|
| Growth protocol |
The zebrafish were maintained at our own facility in a controlled environment with a 14/10-h light-dark cycle at 28 °C. The fertilized eggs were collected at the 1-cell stage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent's Quick Amp Labeling Kit generates fluorescent cRNA with a sample input RNA 1 μg of total RNA for two-color processing.The method uses T7 RNA polymerase,which simultaneously amplifies target material and incorporates cyanine 3- or cyanine 5-labeled CTP.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent Gene Expression Hybridization Kit) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Description |
Biological replicate 2 of 3.
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Apr 01, 2011 |
| Last update date |
Mar 28, 2013 |
| Contact name |
gen hwa lin |
| E-mail(s) |
genhwa@gate.sinica.edu.tw
|
| Phone |
886-2-27899534
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Cellular and Organismic Biology
|
| Lab |
Lab of Marine Molecular Biology and Biotechnology
|
| Street address |
128,section2,academia road
|
| City |
Taipei |
| State/province |
Nankang |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6457 |
| Series (1) |
| GSE28318 |
Zebrafish 22 hpf embryos: WT vs. MO-grnA injected |
|
