|
Status |
Public on Jun 30, 2013 |
Title |
OP21 |
Sample type |
RNA |
|
|
Source name |
Total RNA from PBMCS, nuts, baseline
|
Organism |
Homo sapiens |
Characteristics |
intervention: nuts timepoint: baseline
|
Extracted molecule |
total RNA |
Extraction protocol |
Ultraspec RNA isolation system (Biotecx Laboratories)
|
Label |
Biotin
|
Label protocol |
300 ng of total RNA were amplified and labeled using the 3ยด IVT Express Kit and control reagents (Affymetrix)
|
|
|
Hybridization protocol |
Sample was hybridized to Human Genome U133A 2.0 Array (Affymetrix) using a GeneChip Hybridization Oven 640
|
Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
|
Description |
Gene expression data from Human peripheral blood mononuclear cells
|
Data processing |
The data were analyzed using the RMA algorithm and then SAM was applied to calculate significant differential expression between samples
|
|
|
Submission date |
Apr 04, 2011 |
Last update date |
Jun 30, 2013 |
Contact name |
Olga Castaner |
E-mail(s) |
ocastaner@imim.es
|
Organization name |
IMIM
|
Department |
Nutrition and cardiovascular risk department
|
Lab |
Nutrtion and cardiovascular risk biomolecular laboratory
|
Street address |
Aiguader 88
|
City |
Barcelona |
ZIP/Postal code |
08003 |
Country |
Spain |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE28358 |
Gene expression changes in human peripheral blood mononuclear cells after 3 diet interventions, TMD enriched with olive oil, TMD enriched with nuts and Low fat diet. |
|