|
| Status |
Public on Jun 30, 2013 |
| Title |
IMIM11 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from PBMCS, low fat, 3 months
|
| Organism |
Homo sapiens |
| Characteristics |
intervention: low fat
timepoint: 3 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ultraspec RNA isolation system (Biotecx Laboratories)
|
| Label |
Biotin
|
| Label protocol |
300 ng of total RNA were amplified and labeled using the 3ยด IVT Express Kit and control reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
Sample was hybridized to Human Genome U133A 2.0 Array (Affymetrix) using a GeneChip Hybridization Oven 640
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from Human peripheral blood mononuclear cells
|
| Data processing |
The data were analyzed using the RMA algorithm and then SAM was applied to calculate significant differential expression between samples
|
| |
|
| Submission date |
Apr 04, 2011 |
| Last update date |
Jun 30, 2013 |
| Contact name |
Olga Castaner |
| E-mail(s) |
ocastaner@imim.es
|
| Organization name |
IMIM
|
| Department |
Nutrition and cardiovascular risk department
|
| Lab |
Nutrtion and cardiovascular risk biomolecular laboratory
|
| Street address |
Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE28358 |
Gene expression changes in human peripheral blood mononuclear cells after 3 diet interventions, TMD enriched with olive oil, TMD enriched with nuts and Low fat diet. |
|
