|
| Status |
Public on Feb 01, 2023 |
| Title |
D54-Control-Week1-1 |
| Sample type |
RNA |
| |
|
| Source name |
phNPCs, control, week 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: phNPC
extraction_date: 2022-06-08
expression: Control
replicate: 1
timepoint: Week1
a260: 0.64
a280: 0.298
qubit_conc_ng_per_ul: 21.5
cdna_yield_ug: 151.86
|
| Treatment protocol |
1 microgram/mL doxycycline
|
| Growth protocol |
phNPCs grown in differentation media. See methods of PMID: 36629315.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini Kits (QIAGEN 217004) with the inclusion of an on-column DNase digestion (QIAGEN 79254)
|
| Label |
biotin
|
| Label protocol |
GeneChip 3’ IVT Pico Kit (ThermoFisher 902789)
|
| |
|
| Hybridization protocol |
GeneChip 3’ IVT Pico Kit (ThermoFisher 902789)
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G Plus
|
| Description |
primary human neural progenitor cells grown in differentiation media
|
| Data processing |
Data processed using the rma() function (Robust Multichip Average) using the oligo package in the R programming language.
Clariom_S_Human_HT.r1.pgf
Clariom_S_Human_HT.r1.mps
|
| |
|
| Submission date |
Jan 31, 2023 |
| Last update date |
Feb 01, 2023 |
| Contact name |
Jason L Stein |
| E-mail(s) |
jason_stein@med.unc.edu
|
| Organization name |
UNC Chapel Hill
|
| Department |
Genetics
|
| Lab |
Stein lab
|
| Street address |
Neuroscience Research Building 8109C, 115 Mason Farm Rd
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7250 |
| Country |
USA |
| |
|
| Platform ID |
GPL24324 |
| Series (1) |
| GSE224188 |
Effects of miR-4707-3p expression on differentiation of primary human neural progenitor cells |
|
