|
Status |
Public on Feb 01, 2023 |
Title |
D54-Control-Week1-4 |
Sample type |
RNA |
|
|
Source name |
phNPCs, control, week 1
|
Organism |
Homo sapiens |
Characteristics |
cell type: phNPC extraction_date: 2022-06-08 expression: Control replicate: 4 timepoint: Week1 a260: 0.667 a280: 0.31 qubit_conc_ng_per_ul: 24 cdna_yield_ug: 16.27
|
Treatment protocol |
1 microgram/mL doxycycline
|
Growth protocol |
phNPCs grown in differentation media. See methods of PMID: 36629315.
|
Extracted molecule |
total RNA |
Extraction protocol |
miRNeasy Mini Kits (QIAGEN 217004) with the inclusion of an on-column DNase digestion (QIAGEN 79254)
|
Label |
biotin
|
Label protocol |
GeneChip 3’ IVT Pico Kit (ThermoFisher 902789)
|
|
|
Hybridization protocol |
GeneChip 3’ IVT Pico Kit (ThermoFisher 902789)
|
Scan protocol |
Affymetrix GeneChip Scanner 3000 7G Plus
|
Description |
primary human neural progenitor cells grown in differentiation media
|
Data processing |
Data processed using the rma() function (Robust Multichip Average) using the oligo package in the R programming language. Clariom_S_Human_HT.r1.pgf Clariom_S_Human_HT.r1.mps
|
|
|
Submission date |
Jan 31, 2023 |
Last update date |
Feb 01, 2023 |
Contact name |
Jason L Stein |
E-mail(s) |
jason_stein@med.unc.edu
|
Organization name |
UNC Chapel Hill
|
Department |
Genetics
|
Lab |
Stein lab
|
Street address |
Neuroscience Research Building 8109C, 115 Mason Farm Rd
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599-7250 |
Country |
USA |
|
|
Platform ID |
GPL24324 |
Series (1) |
GSE224188 |
Effects of miR-4707-3p expression on differentiation of primary human neural progenitor cells |
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