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Status |
Public on Jul 01, 2024 |
Title |
cut_tag_h3k27me3_muscle_CBP_0h_rep1 |
Sample type |
SRA |
|
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Source name |
muscle
|
Organism |
Sus scrofa |
Characteristics |
tissue: muscle cell line: C3H cell type: muscle satellite cell genotype: CBP_0h treatment: cell proliferation chip antibody: h3k27me3
|
Treatment protocol |
The cells were treated with 5uM/ml SGC-CBP30 for 48h in the proliferation phase and 60h in the differentiation phase
|
Growth protocol |
C3H cells were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Permeabilized cells in ice-cold NE1 on ice for 10 min, followed by light crosslinking with formaldehyde to fix nuclei. Then, cells nuclei bound to Concanavalin A-coated Magnetic Bead Performed chromatin profiling with CUT&Tag according to its latest protocol
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Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using trimmomatic Trimmed sequence reads were mapped to Sscrofa11.1 genome Peaks were called uing MACS v2.1.0 with the significance cut-off q-value <=0.05 bigWig files were generated using the macs2. narrowPeak files were generated using MACS v2 with default settings. Assembly: Sscrofa11.1 Supplementary files format and content: bigWig, narrowPeak (except for Input sample)
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Submission date |
Feb 02, 2023 |
Last update date |
Jul 01, 2024 |
Contact name |
Xuan Wen Wang |
E-mail(s) |
wwx840994072@gmail.com
|
Phone |
13583396727
|
Organization name |
Huazhong Agriculture University
|
Street address |
No. 1 Shizishan Street
|
City |
Wuhan |
State/province |
Hubei Province |
ZIP/Postal code |
430070 |
Country |
China |
|
|
Platform ID |
GPL26351 |
Series (2) |
GSE224381 |
Epigenetic study on CBP inhibiting differentiation of porcine muscle satellite cells (CUT&Tag) |
GSE224383 |
Epigenetic study on CBP inhibiting differentiation of porcine muscle satellite cells |
|
Relations |
BioSample |
SAMN33023203 |
SRA |
SRX19262151 |