|
Status |
Public on Jul 01, 2024 |
Title |
RNA_muscle_CBP_0h_rep1 |
Sample type |
SRA |
|
|
Source name |
muscle
|
Organism |
Sus scrofa |
Characteristics |
tissue: muscle cell line: C3H cell type: muscle satellite cell genotype: CBP_0h treatment: cell proliferation
|
Treatment protocol |
The cells were treated with 5uM/ml SGC-CBP30 for 48h in the proliferation phase and 60h in the differentiation phase
|
Growth protocol |
C3H cells were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
Extracted molecule |
total RNA |
Extraction protocol |
1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using RNA was harvested using VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina(NR605-02) following manufacturer's protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using trimmomatic Trimmed sequence reads were mapped to Sscrofa11.1 genome Read count extraction and normalization were performed using featureCounts Assembly: Sscrofa11.1 Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
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|
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Submission date |
Feb 02, 2023 |
Last update date |
Jul 01, 2024 |
Contact name |
Xuan Wen Wang |
E-mail(s) |
wwx840994072@gmail.com
|
Phone |
13583396727
|
Organization name |
Huazhong Agriculture University
|
Street address |
No. 1 Shizishan Street
|
City |
Wuhan |
State/province |
Hubei Province |
ZIP/Postal code |
430070 |
Country |
China |
|
|
Platform ID |
GPL26351 |
Series (2) |
GSE224382 |
Epigenetic study on CBP inhibiting differentiation of porcine muscle satellite cells (RNA-Seq) |
GSE224383 |
Epigenetic study on CBP inhibiting differentiation of porcine muscle satellite cells |
|
Relations |
BioSample |
SAMN33023122 |
SRA |
SRX19261983 |