|
Status |
Public on Apr 11, 2023 |
Title |
Cpa3+/+, immunized, 2-bottle test (natural avoidance) day 7, rep2 [AS-929280] |
Sample type |
SRA |
|
|
Source name |
intestine
|
Organism |
Mus musculus |
Characteristics |
genotype: WT (Cpa3+/+) tissue: intestine Sex: male treatment: 2mg alum + 25 µg ovalbumin i.p. on day 0 and 14 + 2 bottle test (water vs. water + 20% egg white + 8% sugar) on days 21-28 time: day7 ova(ag) dlivery: 2-bottle test
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from whole Jejunum (fat and Peyer's plaques removed) according to the instructions of the PureLink RNA Mini kit (Invitrogen) RNA libraries for RNA-seq were prepared using TruSew Stranded RNA Kit (Illumina) following manufacturer's protocols.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 1000 |
|
|
Data processing |
reads were aligned via STAR aligner (default settings, v.2.5.2b) and counted via FeatureCount (default settings, stranded library, subread/1.5.1) Assembly: mm10 (Mus_musculus_GRCm38_102) Supplementary files format and content: text delimeted file includes raw counts for each sample
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|
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Submission date |
Feb 09, 2023 |
Last update date |
Apr 11, 2023 |
Contact name |
Robin Thiele |
E-mail(s) |
robin.thiele@dkfz-heidelberg.de
|
Organization name |
German Cancer Research Center
|
Department |
Immunology
|
Lab |
Cellular Immunology
|
Street address |
Im Neuenheimer Feld 280
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL32159 |
Series (2) |
GSE224895 |
Mast cells link immune sensing to antigen avoidance behavior [intestine] |
GSE225054 |
Mast cells link immune sensing to antigen avoidance behavior |
|
Relations |
BioSample |
SAMN33219723 |
SRA |
SRX19319778 |