|
| Status |
Public on Apr 11, 2023 |
| Title |
Cpa3+/+, immunized, 2-bottle test (natural avoidance) day 16, rep2 [AS-929324] |
| Sample type |
SRA |
| |
|
| Source name |
intestine
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT (Cpa3+/+)
tissue: intestine
Sex: female
treatment: 2mg alum + 25 µg ovalbumin i.p. on day 0 and 14 + 2 bottle test (water vs. water + 20% egg white + 8% sugar) on days 21-37
time: day16
ova(ag) dlivery: 2-bottle test
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from whole Jejunum (fat and Peyer's plaques removed) according to the instructions of the PureLink RNA Mini kit (Invitrogen)
RNA libraries for RNA-seq were prepared using TruSew Stranded RNA Kit (Illumina) following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 1000 |
| |
|
| Data processing |
reads were aligned via STAR aligner (default settings, v.2.5.2b)
and counted via FeatureCount (default settings, stranded library, subread/1.5.1)
Assembly: mm10 (Mus_musculus_GRCm38_102)
Supplementary files format and content: text delimeted file includes raw counts for each sample
|
| |
|
| Submission date |
Feb 09, 2023 |
| Last update date |
Apr 11, 2023 |
| Contact name |
Robin Thiele |
| E-mail(s) |
robin.thiele@dkfz-heidelberg.de
|
| Organization name |
German Cancer Research Center
|
| Department |
Immunology
|
| Lab |
Cellular Immunology
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL32159 |
| Series (2) |
| GSE224895 |
Mast cells link immune sensing to antigen avoidance behavior [intestine] |
| GSE225054 |
Mast cells link immune sensing to antigen avoidance behavior |
|
| Relations |
| BioSample |
SAMN33219718 |
| SRA |
SRX19319783 |
