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Sample GSM7036292 Query DataSets for GSM7036292
Status Public on Oct 26, 2023
Title EC_Dam_1
Sample type SRA
 
Source name EC
Organism Drosophila melanogaster
Characteristics cell line: EC
cell type: Intestinal cells
genotype: MyoIAGal4ts_ UAS_Dam
time: 24h induction at 29°C
Growth protocol Flies were kept at 18°C for crosses and during beginning of adulthood. At 3 day old flies were shifted to 29°C for 1 day to induce Dam transgen expression
Extracted molecule genomic DNA
Extraction protocol For each replicate, DNA was extracted from 60 dissected guts via Qiagen QIAmp DNA Micro Kit following manufacturers' instructions,
Extracted DNA was digested with DpnI, ligated with DamID adaptors, digested with DpnII before PCR amplification. DNA was sonicated to 300bp, adaptors were removed with AlwI and end-repaired and A-tailed. Sequencing adaptors were ligated to the fragments before PCR amplification.
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina HiSeq 2500
 
Data processing damidseq_pipeline script was used to align the reads in fastq files to Drosophila melanogaster reference genome 6.
Each replicate were normalized to its associated Dam alone replicate (log2 ratio of Dam-fusion/Dam alone)
Assembly: Flybase, release 6.13
Supplementary files format and content: bedgraph, gff
 
Submission date Feb 09, 2023
Last update date Oct 26, 2023
Contact name Allison Bardin
E-mail(s) allison.bardin@curie.fr
Organization name Institut Curie
Street address 26 rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL17275
Series (2)
GSE224965 Chromatin state transitions in the Drosophila intestinal lineage reveal principles of cell type specification [DamID]
GSE224967 Chromatin state transitions in the Drosophila intestinal lineage reveal principles of cell type specification
Relations
BioSample SAMN33228644
SRA SRX19323655

Supplementary file Size Download File type/resource
GSM7036292_A1173R01_EC_Dam_1.gff.gz 3.8 Mb (ftp)(http) GFF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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