|
| Status |
Public on Apr 03, 2012 |
| Title |
Frozen Tissue_DASL_66B |
| Sample type |
RNA |
| |
|
| Source name |
Postmortem Brain
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: postmortem brain
tissue preservation: Frozen
diagnosis: Control
assay: DASL
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion MELT kit for frozen brain samples and Roche High Pure FFPE RNA Micro Kit for formalin fixed samples
|
| Label |
Biotin
|
| Label protocol |
DASL- and IVT-based protocols
|
| |
|
| Hybridization protocol |
Illumina hybridization protocol
|
| Scan protocol |
Illumina scanning protocol
|
| Description |
Postmortem brain tissue
|
| Data processing |
The data from frozen-tissue extracted DASL-processed RNA samples passing exclusion criteria were log2 transformed and normalized using quantile normalisation with lumi in R. Quantile-normalized data available on Series record. Raw data included in Sample tables.
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Apr 03, 2012 |
| Contact name |
Maggie Chow |
| E-mail(s) |
mlchow@ucsd.edu
|
| Phone |
858-534-6905
|
| Organization name |
NIH-UC San Diego Autism Center of Excellence
|
| Department |
Neuroscience
|
| Street address |
8110 La Jolla Shores Dr. Ste 201
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE28475 |
Genome-wide Expression Assay Comparison Across Frozen and Fixed Postmortem Brain Tissue Samples |
|
