|
| Status |
Public on Aug 02, 2023 |
| Title |
P422T |
| Sample type |
RNA |
| |
|
| Source name |
breast tumor
|
| Organism |
Homo sapiens |
| Characteristics |
disease: early-stage luminal breast cancer
Sex: female
|
| Growth protocol |
Primary tumor tissue resected from patients
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tumor tissue using the TRIZOL reagent (Thermofisher) following the manufacturer’s protocol. The tissue was first pulverized with mortar and pestle under liquid nitrogen.
|
| Label |
Cy3
|
| Label protocol |
The miRNA Microarray System with miRNA Complete Labeling and Hyb Kit and the SurePrint G3 Unrestricted miRNA 8x60K v19.0 microarray slides (both Agilent Technologies Inc., CA, USA) were used according to the manufacturer’s protocol v4.0. 100 ng of total RNA per sample was dephosphorylated, labeled with Cyanine 3-pCp according to the default manufacturer’s protocol v4.0.
|
| |
|
| Hybridization protocol |
Hybridized and washed using the default manufacturer’s protocol v4.0.
|
| Scan protocol |
Scanned using the Agilent Microarray Scanner instrument with SureScan v9 software (Agilent Technologies Inc., CA, USA). All settings were as recommended by the manufacturer and default for the platform.
|
| Data processing |
Data were extracted using the Feature Extraction software 11.5 (both Agilent Technologies Inc., CA, USA). All settings were as recommended by the manufacturer and default for the platform.
|
| |
|
| Submission date |
Feb 14, 2023 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Petr Holý |
| E-mail(s) |
petr.holy@szu.cz
|
| Organization name |
National Institute of Public Health
|
| Lab |
Toxicogenomics Unit
|
| Street address |
Šrobárova 48/49
|
| City |
Prague |
| ZIP/Postal code |
10000 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL18402 |
| Series (2) |
| GSE225292 |
miRNA transcriptome profiling in early-stage luminal breast cancer |
| GSE225328 |
Transcriptome profiling in early-stage luminal breast cancer |
|
