|
Status |
Public on Feb 18, 2024 |
Title |
51.4.1 vehicle |
Sample type |
protein |
|
|
Source name |
BMDMs
|
Organism |
Mus musculus |
Characteristics |
cell type: bone marrow derived macrophages genotype: C57BL/6 wildtype gender: female treament: vehicle
|
Treatment protocol |
From start of differentiation, cells were kept with 150 ng/ml LCN2 or vehicle.
|
Growth protocol |
Bone marrow was isolated from five wild-type C57BL/6 mice, erythrocytes were lyzed and residual cells were plated in RPMI+10%FCS+1%PenStrep+20ng/ml rmCSF at 3.5x10^6 cells per 10cm dish. Cells were cultivated until d7, when they were treated with LPS and IFNg. 16 hours after stimulation, cell pellets were harvested.
|
Extracted molecule |
protein |
Extraction protocol |
Cell pellets were frozen, shipped, and lyzed in 25 µl buffer with protease and phosphatase inhibitors.
|
Label |
fluorescently labelled anti phospho antibody
|
Label protocol |
maximum 15 µl was used as per proprietary protocol of PamGene
|
|
|
Hybridization protocol |
sample is pumped through porous membrane
|
Scan protocol |
when the solution is underneath the array, images are taken at several exposure times
|
Description |
180-205 29 Protein lysates with active kinases, phosphorylation of spotted peptides on chip detected with fluorescently labeled anti-phospho antibody
|
Data processing |
images are used by the BioNavigator software to calculate signal values for each phosphosite.
|
|
|
Submission date |
Feb 15, 2023 |
Last update date |
Feb 18, 2024 |
Contact name |
Robert Zeiser |
Organization name |
University Medical Center Freiburg
|
Department |
Interdisziplinäres Tumorzentrum
|
Street address |
Hugstetter Straße 53
|
City |
Freiburg |
ZIP/Postal code |
79106 |
Country |
Germany |
|
|
Platform ID |
GPL33127 |
Series (1) |
GSE225341 |
Effekt of recombinant murine Lipocalin-2 (LCN2) on murine macrophages |
|