|
| Status |
Public on Feb 18, 2024 |
| Title |
51.4.4 LCN2 |
| Sample type |
protein |
| |
|
| Source name |
BMDMs
|
| Organism |
Mus musculus |
| Characteristics |
cell type: bone marrow derived macrophages
genotype: C57BL/6 wildtype
gender: female
treament: LCN2
|
| Treatment protocol |
From start of differentiation, cells were kept with 150 ng/ml LCN2 or vehicle.
|
| Growth protocol |
Bone marrow was isolated from five wild-type C57BL/6 mice, erythrocytes were lyzed and residual cells were plated in RPMI+10%FCS+1%PenStrep+20ng/ml rmCSF at 3.5x10^6 cells per 10cm dish. Cells were cultivated until d7, when they were treated with LPS and IFNg. 16 hours after stimulation, cell pellets were harvested.
|
| Extracted molecule |
protein |
| Extraction protocol |
Cell pellets were frozen, shipped, and lyzed in 25 µl buffer with protease and phosphatase inhibitors.
|
| Label |
fluorescently labelled anti phospho antibody
|
| Label protocol |
maximum 15 µl was used as per proprietary protocol of PamGene
|
| |
|
| Hybridization protocol |
sample is pumped through porous membrane
|
| Scan protocol |
when the solution is underneath the array, images are taken at several exposure times
|
| Description |
180-205 38
Protein lysates with active kinases, phosphorylation of spotted peptides on chip detected with fluorescently labeled anti-phospho antibody
|
| Data processing |
images are used by the BioNavigator software to calculate signal values for each phosphosite.
|
| |
|
| Submission date |
Feb 15, 2023 |
| Last update date |
Feb 18, 2024 |
| Contact name |
Robert Zeiser |
| Organization name |
University Medical Center Freiburg
|
| Department |
Interdisziplinäres Tumorzentrum
|
| Street address |
Hugstetter Straße 53
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL33127 |
| Series (1) |
| GSE225341 |
Effekt of recombinant murine Lipocalin-2 (LCN2) on murine macrophages |
|
