|
| Status |
Public on Dec 27, 2023 |
| Title |
myocardium, sham3, 30 min, rep3 |
| Sample type |
SRA |
| |
|
| Source name |
Myocardial tissue
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Myocardial tissue
genotype: WT
treatment: Sham
|
| Treatment protocol |
Male rats were anesthetized, placed supine, and surgically assisted breathing with animal ventilator, and left thoracotomy was performed through a horizontal incision in the fourth intercostal space.
Ischemia (model rat) was observed when the left anterior descending (LAD) coronary artery was ligated around the PE-10 tube with moving nodule with 6-0 sutures, and occlusion of the left anterior descending coronary artery was confirmed by elevation of the ST segment of the electrocardiogram.
|
| Growth protocol |
The rats were placed in a colony room with controlled temperature and humidity, where food and water were freely available in a 12-h light/dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested from the cells using Trizol reagent. TruSeq Stranded Total RNA Library Prep Kit was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Sham3
|
| Data processing |
Paired-end sequencing was performed on cDNA library using Illumina instrument.
The qualified reads were mapped to Rat6 using STAR split mapping algorithm.
The expression level of genes was determined based on the value of FPKM which was calculated by htseq-count.
Assembly: Rat6
Supplementary files format and content: tab-delimited text files include counts and FPKM values for each Sample
|
| |
|
| Submission date |
Feb 15, 2023 |
| Last update date |
Dec 27, 2023 |
| Contact name |
Long Pi Shi |
| E-mail(s) |
sunhongli@hmudq.edu.cn
|
| Phone |
86-459-2796782
|
| Organization name |
Harbin Medical University
|
| Department |
Department of Pharmacology
|
| Lab |
Basic laboratory
|
| Street address |
No.39, Xinyang Road, Longfeng District, Daqing City
|
| City |
Daqing |
| State/province |
Heilongjiang |
| ZIP/Postal code |
163319 |
| Country |
China |
| |
|
| Platform ID |
GPL25947 |
| Series (2) |
| GSE225368 |
Further inhibition of Histone H2A ubiquitination protects myocardium against ischemia/reperfusion injury through enhancing glycolysis and improving mitochondrial function via promoting Hsp27 expression [II] |
| GSE225371 |
Further inhibition of Histone H2A ubiquitination protects myocardium against ischemia/reperfusion injury through enhancing glycolysis and improving mitochondrial function via promoting Hsp27 expression |
|
| Relations |
| BioSample |
SAMN33315888 |
| SRA |
SRX19383398 |
