|
| Status |
Public on Mar 01, 2023 |
| Title |
MCWR_2019_000374 |
| Sample type |
SRA |
| |
|
| Source name |
BMDM
|
| Organism |
Mus musculus |
| Characteristics |
cell line: BMDM
cell type: macrophage
genotype: NOS1 KO
treatment: LPS
time: 8h
|
| Treatment protocol |
Cells were treated at time points with 100 ng/mL LPS.
|
| Growth protocol |
BMDM were generated from bone marrow prepared for in vitro differentiation for 5 days in complete Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco, # 22400089) supplemented with 10% (v/v) fetal bovine serum, 20 ng/mL recombinant murine MCSF (Preprotech, # 315-02), 100 U/mL Penicilin-Streptomycin and 2 mM L-Glutamine, at 37 oC and 5% CO2 atmosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNeasy Mini Kit (QIAGEN, # 74106) with additional DNase treatment using RNase-free DNase Set (QIAGEN, # 1023460).
Sequencing libraries were generated using Illumina Novaseq platform.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
none provided
Assembly: mm10
Supplementary files format and content: tab-delimited text files with htseq counts
|
| |
|
| Submission date |
Feb 22, 2023 |
| Last update date |
Mar 03, 2023 |
| Contact name |
Brian Wray |
| E-mail(s) |
brianwray26@gmail.com
|
| Organization name |
Northwestern University
|
| Department |
Feinberg School of Medicine
|
| Street address |
633 Clark Street
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE225831 |
SOCS1 regulates a subset of NFκB-target genes through direct chromatin binding and defines macrophage functional phenotypes (RNA-Seq). |
| GSE225835 |
SOCS1 regulates a subset of NFκB-target genes through direct chromatin binding and defines macrophage functional phenotypes |
|
| Relations |
| BioSample |
SAMN33415470 |
| SRA |
SRX19471813 |
