|
| Status |
Public on Aug 10, 2023 |
| Title |
Cultured Normal ovarian stromal cells rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Cultured stromal cells, Normal
|
| Organism |
Bos taurus |
| Characteristics |
cell type: cultured stromal fibroblast cells
|
| Treatment protocol |
No specific treatments were given
|
| Growth protocol |
Normal and tumor ovarian stromal cells were cultured in DMEM/F12ꓼ10% FKS media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using innuPREP RNA Mini Kit (Analytik Jena, Germany)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated RNA were prepared according to the standard Affymetrix protocol (GeneChip® Expression 3’Amplification One-Cycle Target Labeling and Control Reagents).
|
| |
|
| Hybridization protocol |
The RNA was hybridized overnight (45°C) in the GeneChipR Hybridization Oven (Affymetrix) to the Affymetrix Bovine Gene 1.0 ST arrays. Washing and staining were performed according to the Affymetrix standard protocol.
|
| Scan protocol |
The scanning of the microarray was done with the GeneChip Scanner 3000 HR (Affymetrix) and image processing was done with the software AGCC (Affymetrix).
|
| Data processing |
Data analysis was performed with TAC 4.0 software (Affymetrix) and RMA method was used for normalization
|
| |
|
| Submission date |
Feb 23, 2023 |
| Last update date |
Aug 10, 2023 |
| Contact name |
Vijay simha Baddela |
| E-mail(s) |
chandu.vijaysimha@gmail.com
|
| Organization name |
Leibniz institute for farm animal biology
|
| Department |
Reproductive biology
|
| Street address |
Wilhelm-Stahl-Allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16500 |
| Series (1) |
| GSE225981 |
mRNA transcriptome data of normal ovarian stromal cells and tumor ovarian stromal cells |
|
