|
| Status |
Public on Oct 11, 2011 |
| Title |
Frogs treated with artemisinin |
| Sample type |
RNA |
| |
|
| Source name |
liver, artemisinin
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: liver
gender: female
treatment: artemisinin
|
| Treatment protocol |
Arteminisin (200 mg/kg) or pregnenolone (50 mg/kg), each dissolved in DMSO, were injected intraperitoneally into three frogs on two consecutive days. The control group received in parallel two DMSO injections. All frogs were sacrificed 24 h after the second injection by decapitation, and livers were immediately frozen in liquid nitrogen.
|
| Growth protocol |
Xenopus laevis were kept in 22°C water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed with peqGOLD TriFast (Peqlab) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on a GeneChip Xenopus laevis 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from artemisinin-treated Xenopus laevis.
|
| Data processing |
The images were processed by GeneChip Operating Software 1.3, and the raw data were normalized by Robust Multiarray Analysis (RMA).
|
| |
|
| Submission date |
Apr 11, 2011 |
| Last update date |
Oct 11, 2011 |
| Contact name |
Marianne Mathäs |
| E-mail(s) |
mariannemathaes@aol.com
|
| Organization name |
Universitätsmedizin Mainz
|
| Street address |
Obere Zahlbacherstraße 67
|
| City |
Mainz |
| ZIP/Postal code |
55101 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE28510 |
Expression data from Xenopus laevis liver |
|
