|
Status |
Public on May 31, 2012 |
Title |
wild type 1 |
Sample type |
RNA |
|
|
Source name |
wt_TP_A
|
Organism |
Drosophila melanogaster |
Characteristics |
age: adult gender: female genotype: wild type genetic background: y1 w67c23 tissue: whole organism
|
Growth protocol |
Flies were cultivated in vials with potato mash-yeast-agar medium.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared from three biological replicates for both wildtype and PofD119. For each replicate, RNA was isolated from 20 adult female flies using TRI-reagent (Ambion) followed by a purification using RNeasy kit (Qiagen) according to the instruction by the suppliers. The six labeled cDNA probes were hybridized to Affymetrix Drosophila Genome 2.0 tiling array.
|
Label |
Biotin
|
Label protocol |
Samples were prepared and hybridized to GeneChip Drosophila Tiling 2.0R Array according to Affymetrix specifications.
|
|
|
Hybridization protocol |
Samples were prepared and hybridized to GeneChip Drosophila Tiling 2.0R Array according to Affymetrix specifications.
|
Scan protocol |
Standard Affymetrix protocol
|
Data processing |
The signal intensity data was analyzed with the Affymetrix Tiling Analysis Software (v. 1.1.02) using parameters: one side upper, 90 bp bandwidth and perfect match only. The resulting CHP file is generated by the Affymetrix Tiling Analysis Software (v. 1.1.02) using parameters: one side upper, 90 bp bandwidth and perfect match only.
|
|
|
Submission date |
Apr 11, 2011 |
Last update date |
Jun 01, 2012 |
Contact name |
Jan Larsson |
E-mail(s) |
jan.larsson@molbiol.umu.se
|
Organization name |
Umea University
|
Department |
Molecular Biology
|
Lab |
Jan Larsson
|
Street address |
Umea University
|
City |
Umea |
ZIP/Postal code |
SE-90187 |
Country |
Sweden |
|
|
Platform ID |
GPL6629 |
Series (2) |
GSE28513 |
Transcriptome profiling of wild type and Pof mutant Drosophila melanogaster females |
GSE28519 |
Pof mutant |
|