|
| Status |
Public on Feb 27, 2024 |
| Title |
Gene Expression KO 10 days |
| Sample type |
SRA |
| |
|
| Source name |
beta cell graft
|
| Organism |
Mus musculus |
| Characteristics |
tissue: beta cell graft
cell line: NIT-1
cell type: beta cell
genotype: Rnls mutant
treatment: syngenic autoreactive splenocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in droplets using the 10X Chromium instrument.
10x Genomics 3' single cell RNA sequencing protocol v3.1 & Cell hashing protocol (https://citeseq.files.wordpress.com/2019/02/cell_hashing_protocol_190213.pdf)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
count_table.csv
metadata.csv
|
| Data processing |
Raw sequencing data were demultiplexed, aligned to the mouse genome (mm10) and UMI-collapsed using CellRanger.
Assembly: mm10
Supplementary files format and content: CSV gene counts
Supplementary files format and content: CSV metadata
|
| |
|
| Submission date |
Mar 01, 2023 |
| Last update date |
Feb 27, 2024 |
| Contact name |
Hui Pan |
| E-mail(s) |
Hui.Pan@joslin.harvard.edu
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics and Biostatistics Core
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE226361 |
Protective Renalase-deficiency in beta cells shapes immune metabolism and function in autoimmune diabetes |
|
| Relations |
| BioSample |
SAMN33554522 |
| SRA |
SRX19538401 |
