|
Status |
Public on Mar 02, 2023 |
Title |
bresat tissue, MULTIseq barcode-derived cDNA, rep 3 |
Sample type |
SRA |
|
|
Source name |
Breast tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: Breast tissue cell type: Multiple cell types library type: MULTIseq barcode antibodies/tags: MULTIseq barcode
|
Treatment protocol |
N/A
|
Growth protocol |
N/A
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Fresh reduction mammoplasty tissue was processed according to Murrow et al (2022) doi: https://doi.org/10.1016/j.cels.2022.06.005 Libraries were prepared using 10X Chromium Next GEM Single Cell 3' Kit v3.1 Single-cell RNA-Seq with MULTIseq workflow
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
Cell Barcode library: read1 file contains cell barcode and molecular index; read2 file contains MULTIseq barcode reads MULTIseq-derived oligonucleotide (HTO)
|
Data processing |
The processed matrices for gene expression and MULTIseq barcode were subsetted to cells classified to the patients described in the study. Assembly: GRCh38-3.0.0 Supplementary files format and content: Folder containing raw FASTQ files for gene expression (RNA) and MULTIseq barcode (HTO) matrices; Folder containing processed matrices for gene expression (RNA) and MULTIseq barcode (HTO)
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|
|
Submission date |
Mar 01, 2023 |
Last update date |
Mar 02, 2023 |
Contact name |
Iain Christopher Clark |
Organization name |
University of California, Berkeley
|
Street address |
374 Stanley Hall
|
City |
Berkeley |
State/province |
CA |
ZIP/Postal code |
94720 |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE202919 |
Microfluidics-free single-cell genomics with templated emulsification |
GSE226391 |
Analysis of cell type heterogeneity in breast tissue II |
|
Relations |
BioSample |
SAMN33560005 |
SRA |
SRX19543308 |