|
| Status |
Public on Mar 15, 2023 |
| Title |
RB355_Etop, INPP4B, rep3 |
| Sample type |
SRA |
| |
|
| Source name |
retinoblastoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: retinoblastoma
cell type: RB355
treatment: etoposide resistant
vector: INPP4B overexpression
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Kit (Qiagen, Hilden, Germany)
Libraries were construced with QuantSeq 3’ mRNA-Seq Library Prep Kit FWD
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Sequences were trimmed with TrimGalore and standard settings
Sequences were aligned with hisat2 (standard settings) on grcm38
Reads were counted by summarizeOverlaps
Assembly: grcm38
Supplementary files format and content: semicolon separated file contains the raw transcript counts
|
| |
|
| Submission date |
Mar 01, 2023 |
| Last update date |
Mar 15, 2023 |
| Contact name |
Bettina Budeus |
| E-mail(s) |
bettina.budeus@uk-essen.de
|
| Organization name |
University Hospital Essen
|
| Department |
Institute for Cell Biology
|
| Lab |
GTF
|
| Street address |
Virchowstrasse 173
|
| City |
Essen |
| ZIP/Postal code |
45147 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE226408 |
Tumor Suppressor Role of INPP4B in Chemoresistant Retinoblastoma |
|
| Relations |
| BioSample |
SAMN33560385 |
| SRA |
SRX19544639 |
