|
| Status |
Public on Mar 01, 2024 |
| Title |
MIA PaCa Ex |
| Sample type |
RNA |
| |
|
| Source name |
pancreatic cancer cell line, exosome
|
| Organism |
Homo sapiens |
| Characteristics |
gender: -
age: -
Stage: -
cell line: MIA PaCa-2
|
| Treatment protocol |
-
|
| Growth protocol |
-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs including miRNAs were isolated and purified from cells using miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
130ng of total RNAs were labeled using the FlashTag™ Biotin HSR RNA Labeling Kit (ThermoFisher), following the manufacturer’s instructions (User manual of the FlashTag™ Biotin HSR RNA Labeling Kit, ThermoFisher)
|
| |
|
| Hybridization protocol |
biotin labeled miRNA was hybridized to ThermoFisher GeneChip miRNA 4.0 array, washed and stained according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner
|
| Data processing |
The data were processed with median polish summarization and quantile normalization by ThermoFisher Transcriptome Analysis Console Software.
|
| |
|
| Submission date |
Mar 02, 2023 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Takahiko Sakaue |
| E-mail(s) |
sakaue_takahiko@kurume-u.ac.jp
|
| Organization name |
Kurume University School of Medicine
|
| Street address |
67 Asahi-machi
|
| City |
Kurume |
| State/province |
Fukuoka |
| ZIP/Postal code |
830-0011 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE226519 |
Pancreatic juice-derived microRNA-4516 and microRNA-4674 as novel biomarkers for pancreatic ductal adenocarcinoma |
|
