|
| Status |
Public on Apr 23, 2023 |
| Title |
Heart_Wt_H2O_3 |
| Sample type |
genomic |
| |
|
| Source name |
Whole heart
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Wildtype (TRα1R384+/+)
gender: Male
age: 4-5 months
housing temperature: 22°C
treatment: Offspring of H2O-treated dam
|
| Treatment protocol |
T3-treated dams received T3 in the drinking water (0.5 mg/L) + 0.01% BSA from embryonic day 12.5 to 18.5, while the drinking water of control dams only contained 0.01% BSA. Offspring were NEVER treated.
|
| Growth protocol |
After mating, female mice were single housed after positive plug check (in order to monitor water/T3 intake as well) at 22°C under constant 12h light/dark cycle with ad libitum access to food and water. Offspring were always group house at 22°C under constant 12h light/dark cycle with ad libitum access to food and water.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extraction was performed with QIAamp DNA Mini Kit (Qiagen, #51304) in accordance to manufacturer´s istructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Mouse Methylation Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
Heart whole methylation data wildtype - offspring of H2O-treated dams
|
| Data processing |
Quality control, background correction by out-of-band (oob) option, dye-bias correction by RELIC and inter-array quantile normalization were performed after exclusion of outliers with the bioconductor package Enmix version 1.34.0
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Mar 06, 2023 |
| Last update date |
Apr 23, 2023 |
| Contact name |
Riccardo Dore |
| E-mail(s) |
riccardo.dore@uksh.de
|
| Organization name |
University of Lübeck
|
| Department |
Institute of Endocrinology and Diabetes
|
| Lab |
Molecular Endocrinology
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| State/province |
Schleswig-Holstein |
| ZIP/Postal code |
23562 |
| Country |
Germany |
| |
|
| Platform ID |
GPL32685 |
| Series (1) |
| GSE226701 |
A cardiac developmental defect prevents thyroid hormone induced tachycardia in the syndrome of resistance to thyroid hormone alpha |
|
