|
| Status |
Public on Mar 13, 2023 |
| Title |
T_I_27 |
| Sample type |
SRA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
cell type: TNBC tumor-bearing mice
treatment: Anti-PD-1 treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (VZ6). 1.5 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Anti-PD-3 treatment in TNBC tumor-bearing mice
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using fastp software
Trimmed sequence reads were mapped to GRCm39 mouse genome
Read count extraction and normalization were performed using RSEM software
Assembly: GRCm39
Supplementary files format and content: tab-delimited xls files include TPM values for each Sample
|
| |
|
| Submission date |
Mar 09, 2023 |
| Last update date |
Mar 13, 2023 |
| Contact name |
Ying Xie |
| E-mail(s) |
sherryyx36@shutcm.edu.cn
|
| Phone |
18017353644
|
| Organization name |
Shanghai University of Traditional Chinese Medicine
|
| Department |
School of public Health
|
| Street address |
725 South Wanping Road, Shanghai
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE226999 |
The impact of PD-1 antibody or G-CSF antibody on the immune system and the hematopoiesis of TNBC mice |
|
| Relations |
| BioSample |
SAMN33698262 |
| SRA |
SRX19610913 |
