|
Status |
Public on Mar 13, 2023 |
Title |
N_P_29 |
Sample type |
SRA |
|
|
Source name |
spleen
|
Organism |
Mus musculus |
Characteristics |
tissue: spleen cell type: TNBC tumor-bearing mice treatment: WT
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Rneasy mini plus kit (VZ6). 1.5 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
WT
|
Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using fastp software Trimmed sequence reads were mapped to GRCm39 mouse genome Read count extraction and normalization were performed using RSEM software Assembly: GRCm39 Supplementary files format and content: tab-delimited xls files include TPM values for each Sample
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|
|
Submission date |
Mar 09, 2023 |
Last update date |
Mar 13, 2023 |
Contact name |
Ying Xie |
E-mail(s) |
sherryyx36@shutcm.edu.cn
|
Phone |
18017353644
|
Organization name |
Shanghai University of Traditional Chinese Medicine
|
Department |
School of public Health
|
Street address |
725 South Wanping Road, Shanghai
|
City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE226999 |
The impact of PD-1 antibody or G-CSF antibody on the immune system and the hematopoiesis of TNBC mice |
|
Relations |
BioSample |
SAMN33698251 |
SRA |
SRX19610906 |