|
| Status |
Public on Apr 15, 2011 |
| Title |
TGFBR1 inhibitor, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Chicken embryo dermal myofibroblasts treated with 500 nM TGFBR1 Kinase Inhibitor II
|
| Organism |
Gallus gallus |
| Characteristics |
breed: brown Leghorn
cell type: chicken embryo dermal myofibroblasts (CEDM)
treatment: TGFBR1 kinase inhibitor II
|
| Treatment protocol |
The cells were treated with 500 nM TGFBR1 Kinase Inhibitor II (2-(3-(6-Methylpyridin-2-yl)-1H-pyrazol-4-yl)-1,5-naphthyridine) from Calbiochem dissolved in DMSO for 48 hours. The control cells were treated with 0.1% DMSO only.
|
| Growth protocol |
The CEDM cells were obtained from day 10 chicken embryo skin explants and cultured in DMEM supplemented with antibiotics, 4% fetal bovine serum and 2% chicken serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from chicken embryo dermal fibroblasts treated with TGFBR1 Kinase Inhibitor II.
|
| Data processing |
The data were analyzed with Partek Genomics Suite (version 6.4) using Affymetrix default annotation and GC-RMA as normalization method.
|
| |
|
| Submission date |
Apr 14, 2011 |
| Last update date |
Apr 15, 2011 |
| Contact name |
Vladimir Cermak |
| E-mail(s) |
cermak@img.cas.cz
|
| Organization name |
Institute of Molecular Genetics, Czech Academy of Sciences
|
| Lab |
Molecular Virology
|
| Street address |
Videnska 1083
|
| City |
Prague |
| ZIP/Postal code |
14220 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE28634 |
Molecular analysis of the TGF-beta controlled gene expression program in chicken embryo dermal myofibroblasts |
|
