|
| Status |
Public on Mar 17, 2023 |
| Title |
ROpos_NoStim_D10_A3225 |
| Sample type |
RNA |
| |
|
| Source name |
Healthy Donor PBMC
|
| Organism |
unidentified |
| Characteristics |
disease state: Healthy
cell type: PBMC
array_id: Hsib-5316848011-D
biosample: A3225
donor: Donor10
pma treatment: NoStim
t-cell subset: ROpos
|
| Treatment protocol |
without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37oC under 5% CO2
|
| Growth protocol |
FACS-sorted naïve, CCR6neg, CCR6low, CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
using RNeasy Mini Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
500 ng of total RNA was used to make cDNA and label cRNA using the Ambion Illumina TotalPrep RNA Amplification kit (Invitrogen).
|
| |
|
| Hybridization protocol |
cRNA samples were hybridized to an Illumina HumanWG-6_V3_0_R3_11282955 BeadChip array for 14-20 hour at 580C in an Illumina Hybridization Oven (Illumina Inc.).
|
| Scan protocol |
Arrays were washed and scanned following the protocols in the Illumina Whole-Genome Gene Expression Direct Hybridization Assay Guide (Illumina).
|
| Description |
not used in final analysis
|
| Data processing |
Signal data were extracted from the image files with the Gene Expression module of the GenomeStudio software (Illumina, Inc.)
|
| |
|
| Submission date |
Mar 16, 2023 |
| Last update date |
Mar 17, 2023 |
| Contact name |
Timothy G Myers |
| E-mail(s) |
tgm@nih.gov
|
| Organization name |
National Institute of Allergy and Infectious Diseases
|
| Department |
Research Technologies Branch
|
| Lab |
Genomic Technologies Section
|
| Street address |
50 South Drive, Room 5509
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-8005 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (2) |
| GSE227487 |
Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticity [BeadChipHumanWG-6] |
| GSE227488 |
Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticity |
|
