|
| Status |
Public on Mar 21, 2023 |
| Title |
WT A549, rSeV-N5R, 4dpi |
| Sample type |
SRA |
| |
|
| Source name |
A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: lung carcinoma epithelial cells
genotype: WT
treatment: rSeV-N5R (MOI=5) --- 4dpi
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol phenol chloroform or Qiagen Rneasy kit
Poly-A enrichment and TruSeq stranded OR TruSeq stranded Total RNA with RiboZero Gold
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
PolyA enriched and prepared using TruSeq Stranded mRNA library prep
|
| Data processing |
Base calling and sample deconvolution performed via Illumina BaseSpace
Host reads aligned to Homo sapiens HG19 reference genome assembly and quantified using Salmon as made available in Illumina BaseSpace "RNA-Seq Alignment" application
Viral reads were aligned to viral reference genome via Bowtie 2 using custom app on illumina basespace, Integrative Genomics Viewer (IGV) 2.15.2 was then used to generate a .csv files comparing individual base calls per aligned read to each nucleotide in the reference sequence
Assembly: hg19
Supplementary files format and content: .quant.genes.sf Salmon files (show raw and normalized read counts by gene)
Supplementary files format and content: .csv files show the read count for individual base calls compared to the viral reference sequence
|
| |
|
| Submission date |
Mar 17, 2023 |
| Last update date |
Mar 22, 2023 |
| Contact name |
Skyler Andrew Uhl |
| E-mail(s) |
skyleruhl@gmail.com
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Microbiology
|
| Lab |
Lim & tenOever labs
|
| Street address |
1 Gustave L. levy Pl.
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE227592 |
ADAR1 biology can hinder effective antiviral RNA interference |
|
| Relations |
| BioSample |
SAMN33799927 |
| SRA |
SRX19713179 |
