|
Status |
Public on Nov 28, 2023 |
Title |
CMP (group B) MXIE |
Sample type |
RNA |
|
|
Source name |
CMP_MXIE_r2
|
Organism |
Mus musculus |
Characteristics |
biological replicate: 2 cell sort information: FACS Sorted BM LKS-CD34+CD16/32- pool: B tissue: Bone Marrow tissue: CMP overexpression??: MXIE genetic background: C57BL/6
|
Growth protocol |
Cell were FACS sorted from primiary receipent mice 16 weeks post transplantation of retrovirally overexpressed immature bone marrow cells.
|
Extracted molecule |
total RNA |
Extraction protocol |
Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
|
Label |
biotin
|
Label protocol |
Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
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|
|
Hybridization protocol |
Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
|
Scan protocol |
Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
|
Description |
Common Myeloid Progenitor MXIE overexpression rep 2
|
Data processing |
samples were RMA normalised in GenePattern using NormalizeAffymetrixST 2.2 (RMA, core probesets, controls included,Affymetrix_MoGene_1_1_st_v1.na33.2.mm9.transcript.chip).
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|
|
Submission date |
Mar 20, 2023 |
Last update date |
Nov 28, 2023 |
Contact name |
Alistair Morgan Chalk |
E-mail(s) |
achalk@svi.edu.au, alistair.chalk@gmail.com
|
Organization name |
St Vincent's Institute of Medical Research
|
Department |
Stem Cell Regulation Unit
|
Lab |
Walkley
|
Street address |
9 Princes st, Fitzroy
|
City |
Melbourne |
State/province |
VIC |
ZIP/Postal code |
3065 |
Country |
Australia |
|
|
Platform ID |
GPL11533 |
Series (1) |
GSE227775 |
Modeling myelodysplastic syndromes in mice by altered Hoxa1 spliceform expression (CMP) |
|