|
| Status |
Public on May 22, 2024 |
| Title |
IonXpress_017_R_2019_10_10_17_02_28_NGS56_libQ201 |
| Sample type |
SRA |
| |
|
| Source name |
ND42222
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ND42222
cell type: hiPSC
genotype: wt/-
treatment: CRISPR
clone: G10
time: Day 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini or micro plus kit (Qiagen). 100ng of mRNA was used for the construction of sequencing libraries.
RNA libraries for 3'RNA-seq were prepared using Qunatseq mRNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
Torrent_Suite 5,6,0
FASTQ file were generated with FastqCreator_jb v3.6.0-r57238 (799) on Torrent Suite
Fastq were import on Partek Flow software (v6 Partek Inc) and analyse using Partek Flow Pipeline for Ion Whole Transcriptome Analysis
differentially expressed genes between groups were quantified using Partek E/M algorithm normalised by the Total count/sample
Assembly: hg19
Supplementary files format and content: tab-delimited text files include RPM values for each Sample
|
| |
|
| Submission date |
Mar 27, 2023 |
| Last update date |
May 22, 2024 |
| Contact name |
MARGOT JARRIGE |
| E-mail(s) |
mjarrige@istem.fr
|
| Phone |
+33169908529
|
| Organization name |
I-Stem/INSERM U861
|
| Street address |
28, Rue Henri Desbruères
|
| City |
Corbeil-Essonnes |
| ZIP/Postal code |
91100 |
| Country |
France |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE228254 |
Mono and biallelic inactivation of Huntingtin gene in patient-specific iPS cells reveal HTT roles in striatal development and in neuronal functions impaired in Huntington’s disease |
|
| Relations |
| BioSample |
SAMN33926897 |
| SRA |
SRX19782197 |
