|
| Status |
Public on Jul 09, 2012 |
| Title |
human pancreatic nontumor tissue, patient sample 20 |
| Sample type |
RNA |
| |
|
| Source name |
non-tumor tissue, patient 20
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: N
survival_month: 23
cancer_death: 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were flash frozen immediately after surgery.RNA from frozen tissue samples was extracted using standard TRIZOL (Invitrogen) protocol. RNA quality was confirmed with the Agilent 2100 Bioanalyzer (Agilent Technologies) before the microarray gene expression profiling
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix GeneChip Hybridization, Wash, and Stain Kit and according to the manufacturer's protocol.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
cancer related death
|
| Data processing |
All arrays were RMA normalized and gene expression summaries were created for each gene by averaging all probe sets for each gene using Partek Genomics Suite 6.5. All data analysis was performed on gene summarized data.
|
| |
|
| Submission date |
Apr 20, 2011 |
| Last update date |
Jul 09, 2012 |
| Contact name |
Perwez Hussain |
| E-mail(s) |
hussainp@mail.nih.gov
|
| Organization name |
NCI/NIH
|
| Street address |
37 Convent Drive
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE28735 |
Microarray gene-expression profiles of 45 matching pairs of pancreatic tumor and adjacent non-tumor tissues from 45 patients with pancreatic ductal adenocarcinoma |
|
