NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM711982 Query DataSets for GSM711982
Status Public on Jul 09, 2012
Title human pancreatic tumor tissue, patient sample 43
Sample type RNA
 
Source name tumor tissue, patient 43
Organism Homo sapiens
Characteristics tissue: T
survival_month: 4
cancer_death: 1
Extracted molecule total RNA
Extraction protocol Tissues were flash frozen immediately after surgery.RNA from frozen tissue samples was extracted using standard TRIZOL (Invitrogen) protocol. RNA quality was confirmed with the Agilent 2100 Bioanalyzer (Agilent Technologies) before the microarray gene expression profiling
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
 
Hybridization protocol Samples were hybridized using Affymetrix GeneChip Hybridization, Wash, and Stain Kit and according to the manufacturer's protocol.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Description cancer related death
Data processing All arrays were RMA normalized and gene expression summaries were created for each gene by averaging all probe sets for each gene using Partek Genomics Suite 6.5. All data analysis was performed on gene summarized data.
 
Submission date Apr 20, 2011
Last update date Jul 09, 2012
Contact name Perwez Hussain
E-mail(s) hussainp@mail.nih.gov
Organization name NCI/NIH
Street address 37 Convent Drive
City Bethesda
ZIP/Postal code 20892
Country USA
 
Platform ID GPL6244
Series (1)
GSE28735 Microarray gene-expression profiles of 45 matching pairs of pancreatic tumor and adjacent non-tumor tissues from 45 patients with pancreatic ductal adenocarcinoma

Data table header descriptions
ID_REF
VALUE RMA normalized gene level expression values from Partek Genomics Suite 6.5

Data table
ID_REF VALUE
8157281 2.82559
7997332 4.48413
8072798 4.93756
7972808 3.58856
8157283 6.83302
7947375 2.07191
7972810 5.89403
8097335 4.92108
8047377 1.8399
7997336 3.37439
8047379 3.79442
8047381 7.27092
8022856 3.14997
8157300 4.86222
8072817 4.86779
7997346 3.70437
8107348 3.7001
8157308 3.4279
8107350 5.88914
8072825 4.64461

Total number of rows: 28869

Table truncated, full table size 447 Kbytes.




Supplementary file Size Download File type/resource
GSM711982.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap