|
| Status |
Public on Sep 17, 2023 |
| Title |
D710T27_RNA-seq_p50_PD-D2-24h |
| Sample type |
SRA |
| |
|
| Source name |
full embryo
|
| Organism |
Bombyx mori |
| Characteristics |
tissue: full embryo
strain: p50
|
| Growth protocol |
~200 p50 diapaused eggs harvest after release 24h at 25°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Eggs were placed in TRIzol™ Reagent from ThermoFisher Scientific before chorions were broken with a piston pellet to allow total RNA extraction.
Sequencing libraries have been obtained using the Illumina stranded mRNA prep ligation protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
RNA-seq
RNA-seq_p50_PD-D2-24h_AH_TPM.xlsx
|
| Data processing |
Library were sequenced on Novaseq.
Adapter sequences were trimmedfrom reads using trimgalore.
RNA-seq reads were aligned to our custom Bmori_v4 genome assembly using STAR_2.7.8a.
Assembly: Bmori_v4
Supplementary files format and content: Excel table file
Supplementary files format and content: bigWig
|
| |
|
| Submission date |
Mar 28, 2023 |
| Last update date |
Sep 17, 2023 |
| Contact name |
Héloïse Muller |
| E-mail(s) |
heloise.muller@curie.fr
|
| Organization name |
Institut Curie
|
| Lab |
UMR3664
|
| Street address |
26 rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL29210 |
| Series (2) |
| GSE228398 |
Unique territorial and sub-chromosomal organization revealed in the holocentric moth Bombyx mori [RNA-seq] |
| GSE228401 |
Unique territorial and sub-chromosomal organization revealed in the holocentric moth Bombyx mori |
|
| Relations |
| BioSample |
SAMN33954981 |
| SRA |
SRX19798782 |
