|
Status |
Public on Jun 22, 2023 |
Title |
Control (MPI) 1 |
Sample type |
SRA |
|
|
Source name |
entire larvae
|
Organism |
Amphiprion ocellaris |
Characteristics |
cell type: entire larvae genotype: WT treatment: DMSO time: 19 days post treatment
|
Treatment protocol |
DMSO control at 0.0001% MPI diluted at 1/1000
|
Growth protocol |
Larvae were maintained in beakers filled with sea water and appropriate treatment and kept at 27°C
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using a Maxwell®16 System with RNA extraction kit (S-Sa) Stranded RNA Lib Prep, Poly-A
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 2000 |
|
|
Description |
PE 2*150, 1.0 Lane(s)
|
Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using fastp v0.23.2 Transcript expression quantification was performed using Salmon v1.7.0 and Ensembl annotations v105 Gene counts were derived using function tximport (with countsFromAbundance="lengthScaledTPM") Assembly: AmpOce1.0 Supplementary files format and content: xlsx file includes estimated counts for each sample
|
|
|
Submission date |
Mar 28, 2023 |
Last update date |
Jun 22, 2023 |
Contact name |
Simon de Bernard |
Organization name |
AltraBio
|
Street address |
30 rue Pré Gaudry
|
City |
Lyon |
ZIP/Postal code |
69007 |
Country |
France |
|
|
Platform ID |
GPL33291 |
Series (1) |
|
Relations |
BioSample |
SAMN33956987 |
SRA |
SRX19801682 |