|
| Status |
Public on Jun 22, 2023 |
| Title |
Control (MPI) 4 |
| Sample type |
SRA |
| |
|
| Source name |
entire larvae
|
| Organism |
Amphiprion ocellaris |
| Characteristics |
cell type: entire larvae
genotype: WT
treatment: DMSO
time: 19 days post treatment
|
| Treatment protocol |
DMSO control at 0.0001%
MPI diluted at 1/1000
|
| Growth protocol |
Larvae were maintained in beakers filled with sea water and appropriate treatment and kept at 27°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a Maxwell®16 System with RNA extraction kit
(S-Sa) Stranded RNA Lib Prep, Poly-A
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
PE 2*150, 1.0 Lane(s)
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using fastp v0.23.2
Transcript expression quantification was performed using Salmon v1.7.0 and Ensembl annotations v105
Gene counts were derived using function tximport (with countsFromAbundance="lengthScaledTPM")
Assembly: AmpOce1.0
Supplementary files format and content: xlsx file includes estimated counts for each sample
|
| |
|
| Submission date |
Mar 28, 2023 |
| Last update date |
Jun 22, 2023 |
| Contact name |
Simon de Bernard |
| Organization name |
AltraBio
|
| Street address |
30 rue Pré Gaudry
|
| City |
Lyon |
| ZIP/Postal code |
69007 |
| Country |
France |
| |
|
| Platform ID |
GPL33291 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN33956984 |
| SRA |
SRX19801685 |
