|
| Status |
Public on Apr 03, 2023 |
| Title |
Sham4-cerebrospinal fluid |
| Sample type |
protein |
| |
|
| Source name |
Cerebrospinal fluid of rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Cerebrospinal fluid
gender: Male
treatment: Untreated
|
| Extracted molecule |
protein |
| Extraction protocol |
none provided
|
| Label |
Cy3
|
| Label protocol |
Cy3-conjugated streptavidin
|
| |
|
| Hybridization protocol |
Centrifuge the Cy3-streptavidin tubule, then add 1.4 ml of sample diluent, mix well, and then quickly centrifuge again. Add 80 ยต l of Cy3-streptavidin to each well, wrap the slide in aluminum foil and incubate it in a dark place. Incubate it on a shaking table at room temperature for 1 hour.
|
| Scan protocol |
The signals (532 nm excitation, 635 nm emission) were scanned and extracted using an InnoScan 300 scanner (Innopsys, Carbonne, France).
|
| Description |
Secreted proteins
|
| Data processing |
Individual array spots were background subtracted locally by subtracting the median background across spot replicated in each sample, and then normalized using two positive controls.
QAR-INF-1 software was used to analyze the data
|
| |
|
| Submission date |
Mar 29, 2023 |
| Last update date |
Apr 04, 2023 |
| Contact name |
Han Li |
| E-mail(s) |
lihanxinyi@126.com
|
| Organization name |
Xinxiang Medical University
|
| Street address |
601 Jinsui Road, Hongqi District, Xinxiang City, Henan Province
|
| City |
Xinxiang |
| ZIP/Postal code |
453003 |
| Country |
China |
| |
|
| Platform ID |
GPL33293 |
| Series (1) |
| GSE228521 |
10 Inflammatory Factors in Cerebrospinal Fluid and Serum of Parkinson's Disease Rats |
|
