|
| Status |
Public on Mar 27, 2012 |
| Title |
Patient 72010; stage IA; AD |
| Sample type |
RNA |
| |
|
| Source name |
Patient 72010
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: non-small cell lung cancer (NSCLC)
patient id: 72010
Stage: IA
subtype: AD
age: 54
gender: Female
|
| Biomaterial provider |
Ramaswamy Govindan
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA, including miRNA from FFPE lung cancer tissue, RNA was extracted with the Ambion RecoverAll kit (Applied Biosystems, Austin, TX). The recovered RNA was DNAse treated and the quantity and quality were evaluated using a Nanodrop ND-1000 (Thermo Scientific) with an inclusion criterion of A260/A280 ≥1.8.
|
| Label |
BIOTIN
|
| Label protocol |
Two hundred ng of total RNA from each sample were labeled and hybridized on Human v2 MicroRNA Expression BeadChips (Cat. no. MI-102–1024; Illumina), according to the manufacturers recommendations (Illumina MicroRNA Expression Profiling Assay Guide).
|
| |
|
| Hybridization protocol |
Fluorescently labelled cDNA PCR products were hybridized to Human v2 MicroRNA Expression BeadChips (Cat. no. MI-102–1024; Illumina), according to the protocols provided by the manufacturer.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4941078005_G
|
| Data processing |
The data were normalised using quantile normalisation
|
| |
|
| Submission date |
Apr 21, 2011 |
| Last update date |
Mar 27, 2012 |
| Contact name |
Yan Lu |
| Organization name |
Medical College of Wisconsin
|
| Street address |
8701 Watertown Plank Rd
|
| City |
Milwaukee |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE29135 |
MicroRNA profiling and prediction of recurrence/relapse-free survival in stage I lung cancer |
|
