|
| Status |
Public on May 12, 2023 |
| Title |
204887840013_R01C01 |
| Sample type |
genomic |
| |
|
| Source name |
B6.F.1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
Sex: female
strain: C57B6/J
Sex: B6
strain: F
subject: 1
|
| Growth protocol |
Mice were housed from 8 to 20 weeks
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
A 2mm cube of fresh liver tissue was homoginzed immediately following sacrifice into Buffer RLT plus with BME. DNA was then extracted using the Qiagen Allprep kit
|
| Label |
Cy5 and Cy3
|
| Label protocol |
500ng of DNA were bisulfite convered using the EZ gold DNA methylation kit
|
| |
|
| Hybridization protocol |
DNA was processed through the Infinum array protocol for hypbridization
|
| Scan protocol |
Beachip arrays were scanned on the Illumina iScan ssytem to produce Idat files
|
| Description |
genomic DNA from a B6 female mouse
genomic DNA from a B6 female mouse
|
| Data processing |
Data were processed using ENMix
Processed data file contains: Average Beta
|
| |
|
| Submission date |
Mar 30, 2023 |
| Last update date |
May 12, 2023 |
| Contact name |
Elizabeth Martin |
| E-mail(s) |
elizabeth.martin2@nih.gov
|
| Organization name |
NIEHS
|
| Street address |
111 TW Alexander Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27713 |
| Country |
USA |
| |
|
| Platform ID |
GPL31950 |
| Series (1) |
| GSE228602 |
Beadchip technology to detect DNA methylation in the mouse faithfully recapitulates whole genome bisulfite sequencing |
|
