|
Status |
Public on May 12, 2023 |
Title |
204887840016_R01C01 |
Sample type |
genomic |
|
|
Source name |
C3.F.5
|
Organism |
Mus musculus |
Characteristics |
tissue: liver Sex: female strain: C3H/HeJ Sex: C3 strain: F subject: 5
|
Growth protocol |
Mice were housed from 8 to 20 weeks
|
Extracted molecule |
genomic DNA |
Extraction protocol |
A 2mm cube of fresh liver tissue was homoginzed immediately following sacrifice into Buffer RLT plus with BME. DNA was then extracted using the Qiagen Allprep kit
|
Label |
Cy5 and Cy3
|
Label protocol |
500ng of DNA were bisulfite convered using the EZ gold DNA methylation kit
|
|
|
Hybridization protocol |
DNA was processed through the Infinum array protocol for hypbridization
|
Scan protocol |
Beachip arrays were scanned on the Illumina iScan ssytem to produce Idat files
|
Description |
genomic DNA from a C3 female mouse genomic DNA from a B6 female mouse
|
Data processing |
Data were processed using ENMix Processed data file contains: Average Beta
|
|
|
Submission date |
Mar 30, 2023 |
Last update date |
May 12, 2023 |
Contact name |
Elizabeth Martin |
E-mail(s) |
elizabeth.martin2@nih.gov
|
Organization name |
NIEHS
|
Street address |
111 TW Alexander Drive
|
City |
Durham |
State/province |
NC |
ZIP/Postal code |
27713 |
Country |
USA |
|
|
Platform ID |
GPL31950 |
Series (1) |
GSE228602 |
Beadchip technology to detect DNA methylation in the mouse faithfully recapitulates whole genome bisulfite sequencing |
|