|
| Status |
Public on Dec 22, 2005 |
| Title |
3-Methylcholanthrene treated Rat (1 Day) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
treated liver excised one day after 3-Methylcholanthrene treatemen
|
| Organism |
Rattus norvegicus |
| Characteristics |
Tissue: Liver
Duration: 1 Day
|
| Treatment protocol |
Rats were treated with 3-methylcholanthrene (MC) (100 umol/kg) in corn oil (CO) (2 ml/kg)or were given equal volumes of CO, i.p., once daily for 4 days.
|
| Extracted molecule |
total RNA |
| Label |
cy5
|
| Label protocol |
http://edge.oncology.wisc.edu/protocols/genisphere50kit.php
|
| |
|
| Channel 2 |
| Source name |
Vehicle-Treated Control
|
| Organism |
Rattus norvegicus |
| Characteristics |
Tissue: Liver
Duration: 1 Day
|
| Extracted molecule |
total RNA |
| Label |
cy3
|
| Label protocol |
http://edge.oncology.wisc.edu/protocols/genisphere50kit.php
|
| |
|
| |
| Description |
Following hybridization, the chips were scanned using Agilent Microarray scanner (model G2565BA (Agilent, Palo Alto, CA), and the data were analyzed using Agilent Feature Extraction, followed by importing the information into MySQL databases. Normalization was performed by the LOWESS method in the Feature Extraction Software. Dye-Swap performed on each array.
|
| Data processing |
this represent the natural log of the average of the trimmed mean for the six replicates on each of the arrays used in the dye swap
|
| |
|
| Submission date |
Aug 22, 2005 |
| Last update date |
Sep 06, 2005 |
| Contact name |
Aaron Vollrath |
| E-mail(s) |
aaron.vollrath@gmail.com
|
| Phone |
(608)262-1209
|
| Organization name |
McArdle Laboratory
|
| Street address |
1400 University Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL2769 |
| Series (1) |
| GSE3175 |
3-Methylcholanthrene treated Rat |
|
