|
| Status |
Public on Dec 31, 2023 |
| Title |
Homo_A549_NC_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
neo-A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: non-small cell lung cancer cell line
|
| Treatment protocol |
EN2 or control Neo retroviral supernatants collected from 293FT packaging cell was used to infect PC9 and A549 cells. The stable cells were selected by genemycin.
|
| Growth protocol |
The cells were cultured in Dulbecco's modified eagle medium (Gibco)for PC9 or F-12K(Gibco Laboratories, USA) for A549 supplemented with 10% fetal bovine serum (Gibco) at 37℃ in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol (Invitrogen, Carlsbad, CA, USA), and a total of 500 ng RNA was used for cDNA synthesis
|
| Label |
biotin
|
| Label protocol |
sample was labeled with biotin by terminal deoxynucleotidyl transferase (TdT)
|
| |
|
| Hybridization protocol |
sample was hybridized to the Affymetrix Clariom S Assay,human (Cat No 902926) for 16-18 h at 45°C. Following hybridization, the microarrays were washed and stained with Streptavidin Phycoerythrin on the Affymetrix Fluidics Station 450
|
| Scan protocol |
Microarrays were scanned using GeneChip® Scanner 3000 7G.
|
| Description |
Gene expression data from non-small cell lung cancer
|
| Data processing |
The data were analyzed with the robust multichip analysis (RMA) algorithm using default analysis settings and global scaling as a normalization method Values are presented according to log2 RMA signal intensity
We defined the genes with a fold-change of more than 1.5 or less than −1.5 and a p-value of less than 0.05 as different expression genes, which were selected to undergo basic analysis
|
| |
|
| Submission date |
Apr 03, 2023 |
| Last update date |
Dec 31, 2023 |
| Contact name |
Yan Li |
| E-mail(s) |
zoeli_swmu@icloud.com
|
| Organization name |
The Affiliated Hospital of Southwest Medical University
|
| Street address |
No. 25 Taiping Street
|
| City |
luzhou |
| ZIP/Postal code |
646000 |
| Country |
China |
| |
|
| Platform ID |
GPL16043 |
| Series (1) |
|
