|
| Status |
Public on Aug 01, 2011 |
| Title |
hybrid at rich media (YPD), 20min with Phenanthroline, Red dye, repeat #2 |
| Sample type |
RNA |
| |
|
| Source name |
S. cerevisiae BY4741 X S. paradoxus CBS432 (ho::kanMX4/ho::nat1 MATa/MATα)
|
| Organism |
Saccharomyces cerevisiae x Saccharomyces paradoxus |
| Characteristics |
organism(s): hybrid
growth protocol: YPD rich medium at 30°C, 20min with Phenanthroline
|
| Growth protocol |
S. cerevisiae, S. paradoxus and their hybrid were subjected to 150 ug/ml of 1,10-phenanthroline at log-phase and sampled after 0, 20, 40 and 60 minutes.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using MasterPure™ Yeast RNA purification Kit (EPICENTRE).
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Amplificarion Kit (LRIAK) plus, two color.
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit.
|
| Scan protocol |
Arrays were scanned using Agilent Microarray Scanner (p/n G2565BA) with a 5 micron resolution and photomultiplier tube (PMT) set to 100. The raw images were analyzed and quantified using Agilent Feature Extraction software.
|
| Data processing |
Raw hybridization intensities were processed by Agilent Feature Extraction software (default settings), log2-transformed, averaged over two replicate probes and corrected for %GC (the correction factor was defined by subtracting the avarage deviation of all probes with similar %GC from the avaerge of all probes).
|
| |
|
| Submission date |
Apr 25, 2011 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Itay Tirosh |
| E-mail(s) |
itay.tirosh@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Molecular genetics
|
| Lab |
Naama Barkai
|
| Street address |
herzl
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL13449 |
| Series (1) |
| GSE28849 |
Coupled evolution of transcription and mRNA degradation. |
|
