|
| Status |
Public on Aug 08, 2023 |
| Title |
Mouse Lung tumor_KrasLA1;DicerS2D_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
primary lung tumor
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
age in weeks: 30w
strain: C57B6J
tissue: lung tumor
genotype: KrasLA1/+;Dicer1S2D/+
|
| Treatment protocol |
No treatment. This are genetically modified mouse models that carry oncogenic Kras G12D (Kras LA1/+); and phosphomimetic Dicer1 wherein serines at positions 1712 and 1836 were replaced to aspartic acid in a oncogenic Kras G12D background (KrasLA1/+;Dicer1S2D/+)
|
| Growth protocol |
30 to 37 weeks old mice from both genotypes were euthanized following IACUC protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lungs were removed from mouse. Then tumors were identified and collected under the dissecting microscope. 50ug of tumors were homogenized in 1 mL of Trizol (Ambion, #15596018). Total RNA was purified following miRNeasy Mini Kit (Qiagen, #217004). 100ng of total RNA was used for nCounter Nanostring miRNA (NS_M_MIR_V1.5) assay.
|
| Label |
miRNA reported and capture probes
|
| Label protocol |
nCounter Nanostring mouse miRNA (NS_M_MIR_V1.5) assay with capture probes which are complementary to miRNAs sequence
|
| |
|
| Hybridization protocol |
Hybridization was performed following nCounter Nanostring miRNA (NS_M_MIR_V1.5) protocol in where miRNA reporter codeset, hybridization buffer and miRNA capture probe set was added to each sample.
|
| Scan protocol |
Nanostring nCounter instrunent
|
| Description |
miRNAs were assayed using Ncounter Nanostring mouse miRNAs v1.5
|
| Data processing |
Results were analyzed in nCounter Analysis Software v4.0. Statistical analysis was performed using two-tailed t-test and Benjamin-Yekutieli for false discovery rate.
|
| |
|
| Submission date |
Apr 04, 2023 |
| Last update date |
Aug 08, 2023 |
| Contact name |
Raisa Reyes-Castro |
| E-mail(s) |
RAReyes2@mdanderson.org
|
| Organization name |
UT Health MD Anderson Cancer Center
|
| Department |
Genetics
|
| Lab |
Swathi Arur
|
| Street address |
6767 Bertner Ave
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL33314 |
| Series (2) |
| GSE228966 |
Phosphorylated nuclear DICER1 promotes open chromatin state and gastric cell fate in lung adenocarcinomas [Nanostring] |
| GSE228968 |
Phosphorylated nuclear DICER1 promotes open chromatin state and gastric cell fate in lung adenocarcinomas |
|
