|
| Status |
Public on May 23, 2023 |
| Title |
PCs, mRNA-derived cDNA donor1_3 |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PBMC
cell type: B cells
library type: mRNA
antibodies/tags: none
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Ex vivo differentiated B cells/PC at day10, day 13 was removed debris by ficoll gradient and following SEC-seq nanovial loading and secretion incubation. And then CITEseq labeling protocol (CG000149). Sorting loaded viable cells before loading on chromian controller.
10X CG00317 next gem single cell 3' (dual index) with feature barcode technology for surface protein
first antibody: anti IgG-PE, second antibody: TotalSeq™-B0911 anti-phycoerythrin (PE) Antibody
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
NextSeq 1000 |
| |
|
| Description |
3' mRNA library: read1 file contains cell barcode and UMI; read2 file contains transcript
poly A RNA
filtered_feature_bc_matrix_105.h5
|
| Data processing |
cellranger demultiplex and mkfastq
cellranger gene count with GRCh38 library
Assembly: GRCh38
Supplementary files format and content: filtered gene count matrix in h5 generated by cellranger.
Library strategy: CITE-seq
|
| |
|
| Submission date |
Apr 05, 2023 |
| Last update date |
May 23, 2023 |
| Contact name |
Rene Cheng |
| E-mail(s) |
rene271828@gmail.com
|
| Organization name |
Seattle children research institute
|
| Street address |
1900 9th AVE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98101 |
| Country |
USA |
| |
|
| Platform ID |
GPL30882 |
| Series (1) |
| GSE229042 |
SEC-seq human plasma cells in nanovials |
|
| Relations |
| SRA |
SRX19900376 |
| BioSample |
SAMN34105610 |
