|
| Status |
Public on Apr 01, 2024 |
| Title |
GCK2 |
| Sample type |
SRA |
| |
|
| Source name |
mycelium
|
| Organism |
Pyricularia oryzae |
| Characteristics |
tissue: mycelium
cell line: Pyricularia oryzae
cell type: Pyricularia oryzae
genotype: WT-Guy11
treatment: CK
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.4 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using Hieff NGS™ MaxUp Dual-mode mRNA Library Prep Kit for Illumina® following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
we use Cutadapt(v1.15) software to filter the sequencing data to get high quality sequence（Clean Data）forfurther analysis .
Thefiltered reads were mapping to the reference genome using HISAT2 v2.0.5
we used HTSeq(0.9.1) statistics to compare the Read Count values on each gene as the originalexpression of the gene, and then used FPKM to standardize the expression.
Assembly: https://fungidb.org/fungidb/app/downloads/Current_Release/Poryzae70-15/
Supplementary files format and content: tab-delimited text files include FPKM values and gene count for each Sample
|
| |
|
| Submission date |
Apr 10, 2023 |
| Last update date |
Apr 01, 2024 |
| Contact name |
jun Zhang |
| E-mail(s) |
2019202023@njau.edu.cn
|
| Organization name |
Nanjing Agricultural University
|
| Street address |
1 Weigang, Nanjing City, Jiangsu Province
|
| City |
Nanjing |
| ZIP/Postal code |
210095 |
| Country |
China |
| |
|
| Platform ID |
GPL32309 |
| Series (1) |
| GSE229299 |
Peroxisome dynamics determines host-derived ROS accumulation and infectious growth of the rice blast fungus |
|
| Relations |
| SRA |
SRX19917950 |
| BioSample |
SAMN34130244 |
