|
| Status |
Public on Sep 05, 2023 |
| Title |
ANT 2 |
| Sample type |
RNA |
| |
|
| Source name |
primary lung cancer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: adjacent nontumor tissues
status after 5 years: Dead
histology: NSCLC
age: 70 years
Sex: Male
Stage: IIIA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from fresh tissue samples by TRIzol Reagent (Invitrogen life technologies) according to the recommended protocols.
|
| Label |
Cy3
|
| Label protocol |
MRNAs were labeled with the Quick Amp Labeling Kit, One-Color (Agilent p/n 5190-0442), according to the manufacturer’s guidelines.
|
| |
|
| Hybridization protocol |
After stopping the labeling procedure, Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242) was used for mRNAs hybridization according to the manufacturer’s instructions. Hybridize at 65°C for 17 hours.
|
| Scan protocol |
The hybridized chips were scanned with an Agilent Microarray Scanner (Agilent p/n G2565BA).
|
| Description |
Agilent Whole Genome Oligo Microarrays (one-color): Prepare the RNA Sample; Total RNA Clean-up and RNA QC; Prepare labeling reaction; Purify the labeled/amplified RNA and labeled cRNA QC; Hybridization; Microarray Wash; Scanning; Extract data using Agilent Feature Extraction Software;
|
| Data processing |
Agilent Feature Extraction Software is used to read the raw intensity of the image. Add the images (.tif) to be extracted to the FE Project; Set FE Project Properties; Check the Extraction Set Configuration; Save the FE Project (.fep) by selecting File > Save As and browse for desired location; Select Project > Start Extracting and export data to txt.
|
| |
|
| Submission date |
Apr 10, 2023 |
| Last update date |
Sep 05, 2023 |
| Contact name |
Neng Jiang |
| E-mail(s) |
jiangneng@sysucc.org.cn
|
| Organization name |
Sun Yat-sen University Cancer Center
|
| Street address |
651 Dongfeng East Road, Guangzhou 510060, P. R. China
|
| City |
Guangzhou |
| ZIP/Postal code |
510060 |
| Country |
China |
| |
|
| Platform ID |
GPL13497 |
| Series (1) |
| GSE229301 |
Rawdata of mRNAs microarray from 3 fresh lung cancer tissues and 3 fresh non-tumor tissues. |
|
