|
| Status |
Public on Apr 11, 2024 |
| Title |
P310s,Sting-KO, replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: hippocampus
genotype: Sting-/-;P310S
Sex: male
age: 9-month
|
| Growth protocol |
Animals were maintained in facilities with a 12-h light/dark cycle, an average ambient temperature of 21°C, an average humidity of 48%, and had free access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hippocampous were removed, flash frozen on dry ice, and total RNA was extracted using TRIzol reagent (Cat#15596018, ThermoFisher Scientific) according to the manufacturer’s instructions.
Total RNA was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
Sting_KO_1
|
| Data processing |
Basecalls were performed using bcl2fastq v2.17 for Novaseq output.
Fastp (version 0.23.1) was used to perform basic statistics on the quality of the raw reads.
HISAT2 (version 2.1.0)
Samtools (version 1.9)
featureCounts (version 2.0.0)
Assembly: GRCm39
Supplementary files format and content: Tab-separated files include raw-count values for each Sample
Supplementary files format and content: Tab-separated files include normalized values for each Sample
|
| |
|
| Submission date |
Apr 11, 2023 |
| Last update date |
Apr 11, 2024 |
| Contact name |
Erlin Wang |
| E-mail(s) |
wangerlin@shsmu.edu.cn
|
| Organization name |
Shanghai Jiao Tong University School of Medicine
|
| Department |
Songjiang Hospital
|
| Lab |
Songjiang Research Institute
|
| Street address |
748 Zhongshan Middle Road, Songjiang District, Shanghai
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
201600 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE229411 |
Microbial infection instigates tau-related pathology via activating neuroimmune cGAS-STING pathway |
|
| Relations |
| SRA |
SRX19932612 |
| BioSample |
SAMN34143115 |
