|
| Status |
Public on Dec 13, 2011 |
| Title |
Metastatic acral melanoma |
| Sample type |
RNA |
| |
|
| Source name |
lymph node
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: lymph node
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIzol (Invitrogen) and eluted in RNAse-free water.
|
| Label |
biotin
|
| Label protocol |
200 ng of each RNA sample was amplified using the Illumina Totalprep RNA amplification kit following the manufacturer’s instructions (Ambion, UK).
|
| |
|
| Hybridization protocol |
Labelled cRNA was subsequently hybri- dised to Illumina Sentrix-human 6 version 2 expression bead-chips
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Samples were variance stabilising transformed and quantile normalised along with 4 other samples in the batch (other samples not included in this series)
|
| |
|
| Submission date |
Apr 28, 2011 |
| Last update date |
Dec 13, 2011 |
| Contact name |
Simon Furney |
| E-mail(s) |
simon.furney@icr.ac.uk
|
| Organization name |
Institute of Cancer Research
|
| Street address |
237 Fulham Road
|
| City |
London |
| ZIP/Postal code |
SW3 6JB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6884 |
| Series (2) |
| GSE28909 |
Genome wide analysis of acral melanoma (Illumina) |
| GSE28913 |
Genome wide analysis of acral melanoma |
|
