|
| Status |
Public on Oct 18, 2023 |
| Title |
B cell DMSO rep3 |
| Sample type |
genomic |
| |
|
| Source name |
B3 DMSO
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
|
| Treatment protocol |
CD43- or CD43+ cells were treated with DMSO or 1 uM GSK343 for 48 hours
|
| Growth protocol |
To separate CD43- from CD43+ cells, washed splenic lymphocytes (5e7) were resuspended in 450 µL FACS buffer and stained with 50 µL CD43 microbeads on ice for 30 min. Cells were washed once, resuspended in 500 µL FACS buffer and passed through an LD column in a VarioMACS separator as per manufacturer’s recommendation. CD43 microbead-labelled non-B cells were eluted from the column by applying the plunger. Purified B cells in the flow-through were incubated in RPMI-1640 supplemented with 10% (v/v) FBS, 55 µM 2-ME, 2 mM L-glutamine, penicillin and streptomycin plus 2 ng/mL IL-4 and BAFF at 37 °C in 5% CO2.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Monarch genomic DNA purification kit
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Mouse Methylation 285k Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
RnBeads/3.17
|
| |
|
| Submission date |
Apr 13, 2023 |
| Last update date |
Oct 18, 2023 |
| Contact name |
Frederick Andrew Dick |
| E-mail(s) |
fdick@uwo.ca
|
| Organization name |
Western University
|
| Department |
Pathology
|
| Lab |
A4-VRL LRCP
|
| Street address |
790 Commissioners Road E
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A 4L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL30650 |
| Series (2) |
| GSE198232 |
Cytosolic pattern recognition receptors respond to EZH2-inhibition induced viral mimicry response that eliminates splenic B cells |
| GSE229684 |
Splenic CD43- B cells exhibit DNA hypomethylation specifically at repetitive elements compared to CD43+ cells |
|
